当AppDomain被卸载的时候，在该环境中的所有资源也将被回收。关于AppDomain的详细资料参考MSDN。下面是使用AppDomain实现动态卸载DLL的代码，

using System.Collections.Generic;
using System.Text;
using System.Threading;
using System.Reflection;
namespace UnloadDll
{
    class Program
    {
        static void Main(string[] args)
        {
            string callingDomainName = AppDomain.CurrentDomain.FriendlyName;//Thread.GetDomain().FriendlyName;
            Console.WriteLine(callingDomainName);
            AppDomain ad = AppDomain.CreateDomain("DLL Unload test");
            ProxyObject obj = (ProxyObject)ad.CreateInstanceFromAndUnwrap(@"UnloadDll.exe", "UnloadDll.ProxyObject");
            obj.LoadAssembly();
            obj.Invoke("TestDll.Class1", "Test", "It's a test");
            AppDomain.Unload(ad);
            obj = null;
            Console.ReadLine();
        }
    }
    class ProxyObject : MarshalByRefObject
    {
        Assembly assembly = null;
        public void LoadAssembly()
        {
            assembly = Assembly.LoadFile(@"TestDLL.dll");           
        }
        public bool Invoke(string fullClassName, string methodName, params Object[] args)
        {
            if(assembly == null)
                return false;
            Type tp = assembly.GetType(fullClassName);
            if (tp == null)
                return false;
            MethodInfo method = tp.GetMethod(methodName);
            if (method == null)
                return false;
            Object obj = Activator.CreateInstance(tp);
            method.Invoke(obj, args);
            return true;           
        }
    }
}